Search for content, post, videos

3D images of sperms binding to the egg surface

Artwork-by-Isha-Raj-and-Luca-Jovine

Researchers at Karolinska Institutet have obtained the first 3D snapshots of a sperm protein attached to a complementary egg coat protein at the beginning of fertilisation.

The study, which reveals a common egg protein architecture that is involved in the interaction with sperm in both mollusc and mammal, is published in the respected scientific journal Cell.

Using X-ray crystallographic data collected at the European Synchrotron Radiation Facility (ESRF), Luca Jovine’s research team at Karolinska Institutet first visualised the sperm-interacting regions of two egg coat proteins, ZP2 in mammals (including humans) and VERL in the marine mollusc abalone (a classic model system of invertebrate fertilisation) . Both of these molecules contain repeated sequences that play a key role in gamete recognition.

“Mammals and molluscs are thought to be separated by 600 million years of evolution, and their sperm receptor proteins are almost completely different in sequence. However, comparison of the structures conclusively demonstrates that ZP2 and VERL repeats share a common 3D architecture”, says Luca Jovine, Professor of Structural Biology at the Department of Biosciences and Nutrition and the Center for Innovative Medicine at Karolinska Institutet.

Subsequently, the research group determined crystal structures of different VERL repeats bound to lysin, the counterpart protein of VERL on abalone sperm. This gave an unprecedented view of how gametes recognise each other in a species-specific way at the beginning of fertilisation.

“Abalone was our system of choice for this investigation, as it is one of the few organisms where cognate egg coat and sperm proteins are known. Moreover, different species of abalone spawn in the open sea but, despite overlapping habitats and breeding seasons, hybrids rarely occur”, says Professor Jovine.

The VERL-lysin complex structures also suggest how lysin opens a hole into the egg coat, allowing sperm to penetrate into the egg.

“Gamete recognition was first compared to a lock and key mechanism more than one hundred years ago. Our study provides the first example of how this is achieved at the very beginning of fertilisation”, concludes Luca Jovine.

Source: Karolinska Institutet

Photographer: Artwork by Isha Raj and Luca Jovine.

Image: Crystal structure of the complex between a domain repeat of egg coat protein VERL (dark pink) and cognate sperm protein lysin (blue).

Leave a Reply

Your email address will not be published. Required fields are marked *

13 − nine =