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Anti-idiotype Antibodies: A Powerful Weapon for PK/ADA Assays

Types of Anti-IDs in PK Assays

Therapeutic monoclonal antibodies have emerged as the fastest-growing class of therapeutics since the mid-1980s from the increasing number of pharmaceutical companies seeking more drug modalities to extend their pipelines. Additionally, biosimilars now have more opportunities to enter the market as patents of some blockbuster monoclonal antibodies begin to expire. Given this context, assessing the safety and efficacy of biotherapeutics has become imperative during the drug-development process. In particular, pharmacokinetic (PK) and anti-drug antibody (ADA) assays are conducted to study and evaluate new drug candidates and biosimilars. For PK/ADA assays, anti-idiotype antibodies (Anti-IDs) are powerful tools to measure the concentration of antibody drugs in patient samples and serve as positive controls for anti-drug antibodies (ADA).

Anti-IDs in Pharmacokinetic (PK) Assays

Pharmacokinetics (PK) describes and characterizes the four different phases of a drug in the human or animal body: absorption, distribution, metabolism, and excretion (also known as ADME). In drug development, PK assays provide essential information on a drug’s interaction with the body, as well as the intensity and duration of its efficacy. For the development of biosimilar drugs, comparative PK assays are required to evaluate potential differences in biological activity to the reference drug.

Based on different binding modes and properties, anti-idiotype antibodies can be classified into three types: antigen-blocking, non-blocking, and complex specifications. Based on these features, different formats of PK assays can be set up to measure free, total, or bound antibody-drug concentrations in serum.

Anti-IDs can be used to detect and quantitate antibody-drug levels in animal or human serum and are critical detection reagents for PK studies. There are a variety of analytical methods for quantitative analysis of antibody drugs, with ELISA as the most commonly used format. In an anti-ID capture ELISA, anti-IDs are coated to a plate and then samples containing the antibody-drug are added to the system. The antibody-drug is then quantified with labeled anti-IDs that specifically bind to the idiotope of the drug.

Anti-IDs in Immunogenicity/Anti-Drug Antibody (ADA) Assays

Immunogenicity assessment involving the detection of anti-drug antibodies (ADA) is a critical step during the development of therapeutic proteins, such as monoclonal antibodies, ADCs, and fusion proteins. In these situations, a multi-tiered testing approach is usually employed. The approach utilizes a sensitive screening assay to identify positive antibody samples, a confirmatory assay to minimize false-positive results followed by a characterization assay to assess the neutralizing capacity of antibodies.

During the whole process, anti-idiotypic antibodies are indispensable reagents. Different assays can be used to detect ADAs, including ELISA, radioimmunoprecipitation assay (RIPA), surface plasmon resonance (SPR), and electrochemiluminescence (ECL). Among these, bridging ELISA is the most commonly used method that allows detection of all isotypes of ADAs (IgG, IgM, IgA, etc.). In a typical bridging ELISA, the antibody-drug is precoated on a plate, and the labeled antibody-drug is incubated with patient samples to test the presence of ADAs. Anti-IDs will be used as positive controls or reference standards for qualitative analysis of ADAs in samples.

Anti-Idiotype Antibody Generation Packages

In order to speed up your drug development, Sino Biological provides a full range of custom anti-idiotypic antibody production services from antigen preparation, anti-idiotype antibody development to detection method establishment and kit development. As well as a team with strong expertise and experience in targeting diverse modalities, including full-length mAb, F(ab’)2, Fab, scFv, VHH, ADCs, bsAbs, and Fc-fusion proteins. Customized anti-idiotypic antibodies can be used to set up pharmacokinetic (PK)/ADA assays to determine specific antibody-drug or ADA levels in samples.

Table 1. List of Anti-Idiotype Antibody Services

Service Deliverables Timeline
Anti-ID Rabbit pAb Service
  • Purified antibodies
  • CoA
2-3 months
Anti-ID Mouse mAb Service
  • Positive clones, 2 tubes cells/clone
  • Purified antibodies, 10mg/clone
  • ELISA antibody pair (optional)
  • CoA
4-6 months

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