Science article - January 2, 2016
Catching an enzyme in the act of catalysis
Previously invisible enzyme structure revealed with protein engineering and NMR. Enzymes are dynamic proteins. They shift, bend and flex as they catalyze a reaction. X-ray crystallography shows their stable forms but not their fleeting, intermediate conformations. To design enzymes for applications such as retrieving high-value molecules from cellulose, we need to understand their transitional states. […]
