In a new publication in “Vaccines” from Department of Immunology and Microbiology, Centre for translational Medicine & Parasitology at Copenhagen University, Attana’s technology has been applied to determine the interaction kinetics between antibodies from vaccinated mice and the pathogen antigen used for the vaccines.
This provides valuable information for creating a vaccine with optimal neutralizing effect and hence a high level of protection for the individuals, states the company.
The interaction kinetics between the mice antibodies and the peptide
In the study two types of vaccines have been compared. A traditional pathogen peptide vaccine and capsid Virus-Like Particles (cVLP) vaccine. In the latter the cVLP is coated with the peptide. The overall result demonstrates the potential of the cVLP platform to create efficient vaccines, states Attana.
Attana technology has been applied to determine the interaction kinetics between the mice antibodies and the peptide. The mice antibodies have been extracted from mice sera and antibodies from several mice have been pooled (mixed) before the kinetic analysis. Thereby the response at a group level can be determined. (It is also possible to analyze on an individual level by analyzing the mice sera directly).
“At Attana, we would like to pinpoint the difference between the two vaccine versions with respect to antibody interaction kinetics, in particular the off-rate. In the picture below, the interaction kinetics for different concentrations are presented. The off-rate of the cVLP vaccine (the right graph) is very slow, almost parallel with the X-axis, whereas in the peptide vaccine, the off-rate has a visible decreasing signal. This shows that the antibodies from the cVLP vaccine stays significantly longer on the peptide compared to the antibodies from the peptide vaccine. The cVLP antibodies thereby efficiently blocks the peptide from interacting with other molecules in the body, hence inhibits the infection,” states the company.
The application and the results are related to Attana Immunity Profiles, where a slow off-rate of an individual’s sera antibodies is one of the necessary parameters to have a good level of protection against a given pathogen.
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